Determining the Region of Rpl 10 crucial for Nmd 3 release in the 60 S Subunit Pathway of Ribosomal Biogenesis

Nop53p is a novel nucleolar 60S ribosomal subunit biogenesis protein.

Ribosome biogenesis in Saccharomyces cerevisiae occurs primarily in a specialized nuclear compartment termed the nucleolus within which the rRNA genes are transcribed by RNA polymerase I into a large 35 S rRNA precursor. The ensuing association/dissociation and catalytic activity of numerous trans-acting protein factors, RNAs and ribosomal proteins ultimately leads to the maturation of the prec...

Genetic Characterization of smg-8 Mutants Reveals No Role in C. elegans Nonsense Mediated Decay

The nonsense mediated decay (NMD) pathway degrades mRNAs bearing premature translation termination codons. In mammals, SMG-8 has been implicated in the NMD pathway, in part by its association with SMG-1 kinase. Here we use four independent assays to show that C. elegans smg-8 is not required to degrade nonsense-containing mRNAs. We examine the genetic requirement for smg-8 to destabilize the en...

Gemfibrozil protect PC12 cells through modulation of Estradiol receptors against oxidative stress

Introduction: Neurodegenerative diseases are progressive disorders that could impair neuronal functions and structures. Oxidative stress and mitochondrial dysfunction are involved in the etiology of neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease and etc. Gemfibrozil is used as a therapeutic drug for hyperlipidemia. It has been shown that gemfibrozil is n...

Histidine methylation of yeast ribosomal protein Rpl 3 p is 1 required for proper 60 S subunit assembly

The NOG1 GTP-binding protein is required for biogenesis of the 60 S ribosomal subunit.

NOG1 is a nucleolar GTP-binding protein present in eukaryotes ranging from trypanosomes to humans. In this report we demonstrate that NOG1 is functionally linked to ribosome biogenesis. In sucrose density gradients Trypanosoma brucei NOG1 co-sediments with 60 S ribosomal subunits but not with monosomes. 60 S precursor RNAs are co-precipitated with NOG1. Together with the nucleolar localization ...